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Return to UV/Visible
Spectrophotometers
Standard Applications
The DU 800 UV/Visible Spectrophotometer comes standard with a complete set of applications that are designed to meet your needs. The intuitive and integrated user interface allows you to move easily between users, applications, and tasks.
Fixed Wavelength
Measures up to 12 wavelengths and reports calculated results.
Key Features
- Data may be taken in Absorbance or Transmittance.
- Up to 12 wavelengths may be selected and used.
- Calculates results with a user-supplied factor or formula.
- Provides a "Large Number" display for the current sample.
Wavelength Scan (Basic)
One or more samples may be scanned at a selected rate, displayed, and printed. Up to 12 scans can be displayed in overlay and multiple scans may be saved in a single file.
Key Features
- Data may be taken in Absorbance or Transmittance.
- Five scan speeds are provided: 120, 240, 600, 1200, 2400 nm/minute.
- Up to 12 scans may be overlaid on the display.
- Automatically measures scans at timed intervals.
- Automatic or manual scaling of x-and y-axis.
- "Zoom" allows the user to select a region of interest.
- "Trace" determines the exact Absorbance or Transmittance values at a given wavelength.
- "Auto Print" prints each scan after data collection is complete.
- "Auto Save" saves each scan after it is taken.
- Multiple scans may be saved in a single file.
- Stored spectra can be added, subtracted or multiplied.
Kinetics/Time
Allows for the simultaneous measurement and analysis of up to 12 rate reactions. Acquisition data and DAbs/min values are displayed as they are collected.
Key Features
- "AutoPrint" prints the kinetic profile after data collection is complete.
- "AutoSave" saves the acquired data after the run is complete.
- Single or multiple blanks may be specified.
- DAbs/min for each sample and time point.
- User may input activity factor and desired activity "units."
- Calculation and display of rate, variance and result for each sample.
- User selected start and end points can be used for one or more cells to determine reaction rates over any time period in the assay.
- Zoom, trace, curve offset, autoscaling and annotation.
Single Component Analysis
Determines the concentrations of unknowns using a standard curve. The standard curve can be optimized by omitting or re-reading standards that are out-of-range. Standards and/or coefficients may be stored separately to be re-used.
Key Features
- Linear regression with zero or non-zero intercept.
- Non-linear (quadratic) regression.
- Use of up to 30 standards (in duplicate or triplicate, if desired).
- Correction for raised or sloping baseline using one or two background wavelengths (net A2, net A3).
- Individual standard selection with ability to omit or rerun standards that are out-of-range.
- Review and/or edit standard curves prior to storage and/or processing of samples.
- Zoom, trace, curve offset, autoscaling and annotation.
- Statistical analysis of sample data; variance and r2.
- Use of up to three replicates for individual samples.
- Dilution correction for each sample.
Nucleic Acid Analysis (Ratios)
Simplifies the acquisition and processing of data for samples containing nucleic acids and proteins. Absorbance readings, ratios and calculated results are available from five different modes.
Key Features
- 260/280 Ratio without background correction.
- 260/280 Ratio with background correction at 320 nm.
- 230/260 Ratio with background correction at 320 nm for greater protein sensitivity.
- Protein and nucleic acid concentrations using coefficients determined by Warburg and Christian.
- The general ratio mode allows the use of selected wavelengths and factors for the calculation of concentrations.
- Automatic peak detection lets you use exactly the analytical wavelengths your sample require.
Performance Validation
Verifies the performance of the spectrophotometer. The results of the tests can satisfy requirements of regulatory agencies for verification of instrument performance.
Key Features
- Each test can be executed by itself or in a group:
- Wavelength Drive Test (Accuracy and Repeatability)
- Resolution Test
- Baseline Flatness Test
- Noise Test
- Stability Test
- Execution of the tests may be automated using the Performance Validation Scheduler.
- Resulting report may be printed and/or saved.
- Tests do not require samples or user interaction.
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