| The Multiwavelength Kinetics mode of
the Kinetics Plus software permits the investigator to collect
kinetics data over a selected wavelength range with
three-dimensional data display and a choice of rate calculation
methods. It is especially useful to observe the disappearance of a
reactant and the appearance of a reaction product. The program then
allows quantitation of these events, using the Enzyme Mechanism
program, to easily characterize complex mechanisms.
Multiwavelength Kinetics is included in the Kinetics Plus Software.
Features
- Data are displayed in 3-D fashion over the selected wavelength
and absorbance range.
- Reaction rate is calculated using linear or auto-linear, first
order or auto-first order methods.
- After data is collected, the user may determine the rate and
method over the desired time interval.
- Any number of blanks and samples, up to 12 total, can be
analyzed and the results stored.
- Blank reading is subtracted from each following sample
readings, if selected.
- A background wavelength may be specified for automatic
correction of each blank/sample reading for a changing baseline.
- Each sample's data may be analyzed, displayed and tabulated at
up to three wavelengths.
- Interval time between successive scans on a sample is 0.1
second to 9999.9 seconds/minute.
- Total time for the analysis is adjustable from 0.1 second to
9999.9 seconds/minute.
- Read average time may be adjusted from 0.1 to 99.9 seconds.
Ten sets of data are collected every second.
- Up to 12 samples may be analyzed.
- Up to 99 scans may be taken and stored for each sample.
- Data may be reviewed and enhanced with functions as
split/overlay, zoom, trace, autoscale, axis limit changes, and
annotate.
- Cell match function provides automated zero correction of
multiple cells.
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Click on the thumbnail to
view the full-size image in a
separate window.
Three-Dimensional
Spectra Window.
Multiwavelength
Results Window.
Raw Data for Analytical
Wavelength, Background
Wavelength and Corrected
Absorbance.
Initial Rate Calculation
Using Linear Mode.
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