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Enzyme Activity
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There are many instances where enzyme activity measurements in large number of samples are required. These include assays of chromatography column fractions in the purification of an enzyme, quantitation of fusion protein products, and promoter analysis with gene fusion to an enzyme. The last two examples are commonplace in molecular biology laboratories. The DUŽ Series 600 and 7000 Spectrophotometers have incorporated features necessary to greatly simplify and expedite your studies.

With the DU Series 7000, Enzyme Activity is included in the Kinetics Plus Software.

Features

  • Individual selection of analytical and background wavelength.
  • Background wavelength factor may be a constant or a function of wavelength if background changes with wavelength.
  • One or two time points may be chosen for the assay.
  • User may input activity factor and desired activity "units".
  • Replicate assays, up to three.
  • Sample data and/or results may be viewed on screen, printed or saved to memory.
  • Raw and/or reduced data may be transferred via the RS-232 interface.
  • Data and results files may be saved or transferred in ASCII or Lotus 1-2-3 format on a diskette (option).
  • Cell match function provides automated zero correction of multiple cells.
     

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Samples Window Showing
Absorbance Data.

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Results Window Showing
Calculated Rate.

For more information:

T-1703 End Point Kinetic Assays for Determination of Enzyme Activity with the DU Series Spectrophotometers
 

 
 
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