| The Enzyme Mechanism software provides
for rapid and easy characterization of a wide variety of enzyme
reactions with just the click of a mouse button. DU®
Series 600 and 7000 Spectrophotometers with this option will
calculate Km, Vmax, kcat and Ki as well as the Hill constant. Up to
four data plots can be displayed simultaneously (Michaelis-Menten,
Lineweaver-Burk, Eadie-Hofstee, Hanes-Woolf, Hill and Inhibitor
plots) without the need for a computer or external software
packages. The use of this software allows easy determination
and/or confirmation of competitive, non-competitive or uncompetitive
enzyme reaction mechanisms.
With the DU Series 7000, Enzyme Mechanism is included in the
Kinetics Plus Software.
Features
- Automatic blank subtraction and background correction for up
to 24 blanks and samples, at user specified cell positions in the
6 or 12 position cell holder.
- Blanks may be viewed or selected for subtraction.
- Reaction rates can be determined automatically by the
instrument or from user selected time points.
- Choice of rate calculation methods: linear, auto-linear, first
order, and auto-first order.
- Delta A/minute, concentration and enzyme activity
calculations.
- Km, Vmax, kcat, and Ki determinations by Marquardt nonlinear
regression analysis.
- Hill constant calculation.
- Plotting functions include split screen and overlay, zoom,
curve offset, annotate and trace features.
- Data may be averaged from 0.05 to 99.9 seconds (DU Series 600)
or 0.10 to 99.9 seconds (DU Series 7000).
- Calculation of Ki for competitive, uncompetitive and
noncompetitive mechanisms.
- Plots generated include:
- Michaelis-Menten
- Lineweaver-Burk
- Eadie-Hofstee
- Hanes-Woolf
- Inhibition
- Hill
- Sample directly to and from 96-well plates with just 100 µL of
sample.
- Interval time between successive readings on a single sample
may be set from 0.1 to 9999.9 seconds.
- Selectable read average time to improve single-to-noise ratio.
- Rates and concentrations may be entered from external studies
to take advantage of the Enzyme Mechanism Software's capabilities.
- Data from up to 10 sets of 24 samples may be accumulated and
analyzed.
- Raw and/or calculated data may be output to external devices.
- Method parameters and sample data can be stored in separate
files in the instrument for easy recall and use.
- Cell match function provides automated zero correction of
multiple cells.
|
Click on the thumbnail to
view the full-size image in a
separate window.
Michaelis-Menten Plot.
Lineweaver-Burk Plot.
Hanes-Woolf Plot.
Eadie-Hofstee Plot.
Km, Vmax and Kcat Tabulation Window.
Selection of
Michaelis-Menten,
Lineweaver-Burk,
Hanes-Woolf, and
Eadie-Hofstee Plots
on One Screen.
Hill Plot and Constants
for Two Data Sets.
Inhibitor Plot; Competitive Inhibitor.
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