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Kinetic Enzyme/Time
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The built-in Kinetic Enzyme Software (Kinetics/Time) allows for the simultaneous measurement and analysis of up to 12 rate reactions. Data are reviewed in real time, plotted or tabulated as they are collected, and stored in the instrument memory. The rate of the kinetic reaction is calculated using linear regression for the best fit straight line. Results may be printed in both graphic and tabular formats.

Features

  • Real time graphical display of data.
  • Automatic data storage for later replot and calculations.
  • Single or multiple blanks may be specified.
  • Analytical and background correction; wavelengths can be selected.
  • Display of net absorbance, corrected absorbance, rate and calculated concentrations from corrected absorbances.
  • User may input activity factor and desired activity "units."
  • Calculation and display of rate, variance and enzyme activity.
  • Interval times available from 0.1 second to 9999.9 minutes between successive readings of the same cell.
  • Data may be averaged from 0.05 to 99.9 seconds (DU® Series 600) or 0.10 to 99.9 seconds (DU Series 7000).
  • Total time of analysis may range from 0.1 second to 9999.9 minutes.
  • User selected start and end points can be input for collected data from one or more cells to determine reaction rates over any time period in the assay.
  • Zoom, trace, curve offset, autoscaling and annotation for customizing plotted data.
  • Method parameters and sample data can be stored in separate files in the instrument for easy recall and use.
  • Cell match function provides automated zero correction of multiple cells.

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Real Time Data Plotting
Windows for Kinetic
Enzyme Analysis.

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Data Tabulation Window for Kinetic Enzyme Analysis.

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Rates and Results Window
for Kinetic Enzyme
Analysis with Initial Rates.

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Rates and Results Window
for Kinetic Enzyme
Analysis with
Concentrations Determined and Plotted.

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Raw Data Window for
Kinetic Enzyme Analysis.
 

Accessories
The Auto 6 Cell Holder is an automatic sampling accessory that holds up to six standard or Semi-Microcells of 10 mm path length and 4 mm path width. The cell holder is available in three different configurations; unheated, water-regulated and Peltier electronic temperature control.

The Micro-Auto 12 Cell Holder is a water-regulated temperature controlled sampler that holds three Multi-Microcells for up to 12 samples of 100 µL volume each.

For more information:

T-1703 End Point Kinetic Assays for Determination of Enzyme Activity with the DU Series 7000 Spectrophotometers
T-1714 Determination of Multiwavelength Kinetics on Microvolume Samples in Microtiter Format with the DU Series 7000 Spectrophotometers
 

 
 
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