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High-Temperature Micro Kinetics Analysis
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The ability to accurately control the temperature of microvolume samples has given rise to various spectroscopic applications. The most common application is that of Thermal Melt (Tm) for which Beckman Coulter has a dedicated Tm accessory and software. Often a somewhat more flexible system is required for different types of temperature studies such as:
  • Studies of Thermophilic Proteins and Enzymes
  • Enzyme Engineering
  • Fermentation Studies

The High-Temperature Micro Kinetics system is similar to the Tm Analysis system and can be used to accurately control the temperature of six microcells, each containing only 325 µL of sample. The microcells are open-top cells for easy sample handling. The cell holder cover seals the cells to avoid sample evaporation. The High Temperature Micro Kinetics system can be used with all the software available on the DU® 600/7000 Spectrophotometers including Kinetics Time/Drive, Enzyme Mechanism, Enzyme Activity, and Tm Analysis.

Features

  • Peltier temperature-controlled Micro Tm Cell Holder.
  • Integrated temperature sensor; no external temperature probe required.
  • Efficient heat transfer and accurate temperature control is achieved by the use of two high-performance Peltier elements, low-mass cells and optimally positioned temperature sensor.
  • Excess heat is quickly removed by the fan in the transport; no water cooling required.
  • Purge connector to purge cells with nitrogen; required when working below room temperature to prevent condensation on the surface of the cells.
  • Specially designed 1 cm path length microcells for sample volumes of only 325 µL.
  • Cell cover seals automatically to avoid sample evaporation -- no need for oil.
  • High-performance temperature controller with a temperature range from 4º to 110ºC.
  • Compatible with Kinetic Time/Drive, Enzyme Kinetics, Enzyme Activity, and Tm Analysis applications software.

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High-Temperature Micro
Kinetics Cell Holder with
325 ?L Microcells.

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Schematic of High-
Temperature Micro Kinetics
Cell Holder.

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Real-Time Data Plotting
Window of Kinetics
Time/Drive; Refer to the
Appropriate Software for
Data Analysis Features.

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Real-Time Data Plotting
Window of Enzyme
Mechanism; Refer to the
Appropriate Software for
Data Analysis Features.
 

Accessories

The Virtual Disk Expansion (P/N 514252) is recommended when running analyses over a wide temperature range with small time intervals. The External Storage Device (P/N 514244) allows you to archive data for later recall or to transfer files to a computer.
 

 
 
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