| The most common method of studying
denaturation of DNA involves increasing the temperature of a DNA
solution so that the DNA gradually denatures by strand separation.
Renaturation studies can be done simply by reversing the process.
The thermal melting point (Tm) is defined as the temperature by
which 50% of the base pairs of a duplex are separated. All
components of the Micro Tm Analysis Accessory were designed to
perform Experimental Tm Analysis exclusively to provide simplicity
and convenience, small sample volumes, effective heat transfer,
accurate temperate control and high sample throughput.
Denaturation and renaturation studies can be performed with up to
six 325 µL samples at one or two wavelengths. The systems provide
three calculation methods, first derivative, two-point average and
non-linear fit.
Features
- Peltier temperature-controlled Micro Tm Cell Holder.
- Integrated temperature sensor; no external temperature probe
required.
- Efficient heat transfer and accurate temperature control is
achieved by the use of two high-performance Peltier elements, low
mass cells and optimally positioned temperature sensor.
- Excess heat is quickly removed by the fan in the transport; no
water cooling required.
- Purge connector to purge cells with nitrogen; required when
working below room temperature to prevent condensation on the
surface of the cells.
- Specially designed 1 cm path length microcells for sample
volumes of only 325 µL.
- Cells are individually stoppered to avoid evaporation - no
need for oil.
- High-performance temperature controller with a temperature
range from 4 to 110ºC.
- Select one or two analytical wavelengths, or one analytical
and one background wavelength.
- Ramp rate selection from 0.1 to 5.0ºC/minute.
- Up to three temperature ramp rates per analysis, including
read interval for each ramp and delay time.
- Each position in the cell holder can be assigned either to a
sample, blank or marked as empty.
- Reverse experiment and dwell time selection.
- Three calculation methods: first derivative, two-point average
and non-linear fit.
- Report can be customized to include data and plots.
- Data can be saved, recalled and results recalculated.
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Click on the thumbnail to
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Micro Auto 6 Tm Cell Holder with Stoppered 325 ?L Tm Microcells.
Schematic of Micro
Auto 6 Tm Cell Holder.
Main Screen Showing Five Temperature Profiles;
Blank is Hidden.
Temperature Control
Setup Window.
First Derivative
Calculation Mode.
Two-Point Average
Calculation Mode.
Non-Linear Fit
Calculation Mode.
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