The Nucleic Acid Analysis software automates the acquisition and processing of data for samples containing nucleic acids and proteins. Absorbance readings, ratios and calculated results are available from five different programs with or without correction for scattering due to turbidity.

• Traditional 260/280 and 280/260 ratios with no background correction.
• 260/280 and 280/260 ratios with background correction at 320 nm.
• 230/260 ratios with background correction at 320 nm for greater protein sensitivity.
• Protein and nucleic acid concentrations using coefficients determined by Warburg and Christian.
• A general ratio method allows user selected wavelengths and factors for calculation of concentrations of DNA, RNA or oligonucleotides and other ratios.
• Automatic peak detection lets you use exactly the analytical wavelength your sample requires.
• Method parameters, standards calibration data and sample data can be stored in separate files in the instrument for easy recall and use.

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view the full-size image in a
separate window.

Nucleic Acid Samples Window; 260/280 and
280/260 Methods with
Background Correction.

Nucleic Acid Samples Window; Warburg and Christian Concentration Determination.

Formula Setup Window; Warburg and Christian Concentration Determination.
 

The DNA/RNA/Oligo Quantitation mode adds capabilities that automate the acquisition and processing of data from oligo DNA/RNA samples and determines molecular weight, absorptivity (extinction coefficient), concentration, and theoretical melting temperature for oligo DNA samples. Additionally, it simplifies the quantitation of single- and double-stranded DNA and RNA to include corrections for sample dilution and cell path length.

• Quantitation of long oligo DNA and RNA samples (> 30 mers) from base composition.
• Quantitation of short oligo DNA and RNA samples (< 30 mers) from sequence.
• The DNA oligo modes allow you to determine the theoretical thermal melting point (Tm) for DNA oligonucleotide samples.
• Preconfigured methods let you quantitate single- and double-stranded DNA and RNA samples quickly and easily.
• The double ratio method (e.g., 260/280 and 260/230) provides an even better way to determine the purity of your samples; with and without nucleic acid quantitation.

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view the full-size image in a
separate window.

DNA Oligo Long; Determination of Extinction Coefficient, Concentration
and Theoretical Melting
Point (Tm) with Entered
Base Composition.

DNA Oligo Short; Determination of Extinction Coefficient, Concentration
and Theoretical Melting
Point (Tm) with
Entered Sequence.

Double-Stranded DNA Determination Window.

Double Ratio Window.
 

For highest throughput with best sensitivity and no need to remove the sample cell for flushing or cleaning, the 50 µL Aspiratable Sipper system should be your choice.
 

 

50 µL Microcell and 5 µL
Ultra-Microcell and their
Respective Cell Holders.