|
Managed by: Dr. Peter Schuck
(pschuck@helix.nih.gov)
Available at:
Sedfit
This is software for the analysis of analytical ultracentrifuge data
files by direct fitting with numerical solutions of the Lamm Equation,
Van-Holde-Weischet analysis, and ls-g*(s) and c (s) analysis. c (s)
analysis is possible with several variants for using prior knowledge
(e.g. conformational change models), or in general model that
reveals a weight-average frictional ratio of the molecules. For the
discrete Lamm equation fitting, different models are implemented, currently for
up to four ideally sedimenting components, several self-association
schemes, and non-ideal sedimentation.
All systems are implemented with finite element methods,
including a new moving frame of reference method, automatically
selecting the optimal method for rapid fitting. Sedfit allows the
explicit algebraic calculation of time-invariant and
radial-invariant systematic noisecomponents, for use with
interference data. Also, it can be used for extracting
time-invariant noise contributions from approach-to-equilibrium
scans, for correcting the sedimentation equilibrium profiles.
Sedfit can take experimental scans as initial distributions, which is
useful in synthetic boundary experiments, or if the acceleration phase
of the experiment is significant in the sedimentation process.
Several methods for statistical analysis of the fit and for
calculation of confidence intervals of the derived parameters are
implemented. As general utilities, several data conversion functions,
and methods to eliminate integral fringe shifts from individual scans or
from sets of scans, are available, as well as analogous
size-distribution models for the analysis of dynamic light scattering
data.
The website contains a detailed help for getting-started, tutorials
on several topics, and the complete on-line help reference for Sedfit.
|
