• Homogenates
• Coarse Ground Tissue
• Organs

Homogenates

1. Add 0.2 mL¹ of up to 10% tissue homogenate (in either water or 70% ethanol) to 1 mL of water.
2. Add 10 mL of Ready Gel™ or Ready Safe™, shake vigorously and count.

Notes:

1. Homogenates of up to 1 mL can be processed by following the procedure for coarse ground tissue below.

Expected tritium efficiency is 35-40%.

For homogenate assays that include a filtration step on glass fiber filters see Part 4 - Inert Supports.

Coarse Ground Tissue

1. Add 150 mg of coarse ground tissue to 2 mL of BTS-450 and swirl.
2. Incubate 3-5 hours at 40ºC.
3. Add 10-15 mL of Ready Organic™ containing 7 mL glacial acetic acid per liter of cocktail¹.
4. Shake and count.

Note:

1. See section on reducing chemiluminescence.

Organs

1. Add 1 mL BTS-450 to the tissue weights shown in the following table:
    

   Sample	Amount		Sample	Amount
   Arteries	20-100 mg		Liver	80-100 mg
   Brain	80-130 mg		Muscle	100-220 mg
   Cartilage	20- 55 mg		Nerve Cells	50-100 mg
   Cornea	40-160 mg		Pancreas	50-140 mg
   Heart	100-120 mg		Spleen	50-140 mg
   Intestine	80-100 mg		Stomach	80-100 mg
   Kidney	80-100 mg

2. Incubate 2-4 hours at 40ºC or until solubilized.

3. Add 10-15 mL of Ready Organic containing 7 mL glacial acetic acid per liter of cocktail¹.

4. Shake and count.

Note:

1. See section on reducing chemiluminescence.
    

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