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T3 Update Online -- Tips, Tricks and Techniques for Users of Beckman Coulter Life Science Automation |
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Welcome From the Editors -- Fall 2007 Issue
Welcome to the Fall 2007 Issue of T3 Update Online. In this issue, we offer a bit of something for everyone! We proudly present the Biomek®3000 ArrayPlex application, which has been awarded Premier Application Status from Affymetrix. And, we introduce a new chemistry Agencourt® Chloropure™, which purifies RNA and DNA from plant material.
Because of the strong interest you’ve shown in pipetting performance verification, we have teamed with ARTEL* and, in this issue, we show you how to ensure top performance from your liquid handler. Plus, for those of you who like the convenience of shopping online, we offer information about our new Beckman Coulter eStore.
For those who crave technical details, we’ve got lots in that area too. We’ve provided a link to the Club Biomek User Forum, and present you with a great new SAMI® Tricks and Tips article. Add to this several application posters and user presentations and the result is an exciting and highly informative issue of T3 Update Online.
Enjoy the issue and please continue to let us know what interests you.

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Marc Goldstein, Ph.D., Mary X. Guan and Keith Roby. Learn more in the "About the Editors" section.

Download a printable version of the T3 Update On-Line (PDF Format, 1.73 MB).
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New Chemistry |
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Introducing Agencourt® Chloropure™
The Agencourt Chloropure system utilizes Agencourt’s patented Solid Phase Reversible Immobilization (SPRI®) paramagnetic bead-based technology to rapidly isolate both DNA and RNA from plant material. This extraction kit is highly amenable to automation by using the Beckman Coulter Biomek® laboratory automation workstations. more 
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More News |
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Qualifying Beckman Coulter Liquid Handlers With the MVS* Verification System From ARTEL (Online Webinar available now!)On August 21, 2007, Beckman Coulter and ARTEL technical experts gave a webinar to discuss the standardized ARTEL MVS method of qualifying both accuracy and precision on Biomek® laboratory automation workstations. Click to view the webinar. more |
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ArrayPlex applications for Automated Target RNA Preparation on the Biomek 3000 Laboratory Automation Workstation Has Received Affymetrix's Premier Applications Provider Status
This award enables Beckman Coulter to provide an automated target RNA Preparation solution on the Biomek 3000 for Affymetrix’s customers. To achieve Premier Application Provider Status, Beckman Coulter has demonstrated that it meets Affymetrix' high quality standards. The ArrayPlex 3’ IVT application for automated RNA target prep on the Biomek 3000 system offers the same high level of consistency, flexibility, scalability and reduced FTE benefits as the Biomek FXP, but on an instrument with a more compact footprint and cost-effectiveness. The ArrayPlex application includes automated instrumentation, software and protocols on the Biomek 3000 using Agencourt’s RNAClean™ chemistry for post-reaction cleanup. more |
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Simplify Your Ordering Process With Beckman Coulter’s New eStore
With Beckman Coulter’s new eStore, you now have week access to Beckman Coulter’s wide array of products, accessories and reagents 24 hours/day, seven days/week. You can order directly or request a quote **. Account-specific pricing is automatically provided when you log in. Plus, your research automation consumables, including Biomek® disposable pipette tips, are as easy as ever to order directly online. Stop in to see how easy shopping with us can be. Click link for the Research Automation in eStore.
** Online ordering available only in certain countries (U.S., France, Germany and U.K.). Request for quote is available in all countries. |
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Agencourt CleanSEQ® Protocol Version 32
Agencourt has recently updated the Agencourt CleanSEQ protocol to version 32 from version 31. The new revision includes more details in the description of the steps to make it easier to read. Click here for further details on the content of this letter (PDF). |
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New Tips |
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Tips From Club Biomek Forum
In this issue, we introduce you to one of the best resources for Biomek-related information: Club Biomek Forum on the web (http://forums.beckmancoulter.com)! In addition, we provide you a brief FAQ about Club Biomek. Within each issue of T3, we will highlight a few topics of interest from the web forum. This time, we'll focus on two threads of interest, both from the "scripting" genre. more  |
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SAMI® Tips and Tricks
T3 newsletter has always been committed to providing you the most up-to-date tips and frequently asked questions and answers. In this issue, Christoph Krüll, Ph.D, from Beckman Coulter, shares with you how to use MD assign module in SAMI software to log all three generations of barcodes in the assay plates’ reader log file. more  |

Genomics
- Automated Affymetrix Human Mapping 500K Target Preparation on Biomek® FX Laboratory Automation Workstation Handy Yowanto, Noel Ruppert, Debbie Benitez, David J. Duggan, William Biggs and Cynthia French. more


Drug Discovery
- Use of a 10-Cytokine Multiplex RT-PCR in Conjunction With a Corresponding 10-plex Fluorescent Bead Quantitative Protein Immunoassay to Reveal Expression Patterns in Mitogen-Stimulated Human Peripheral Leukocytes Cynthia Boardman, Yu Suen, Keith Roby and Scott Boyer. more


Application Posters Conference Recaps - Second Europe Biomek® User Summit
Summit Overview
- The 2nd European Biomek® User Summit took place in Divonne, France, June 4-5, 2007. The Summit began with a selection of hands-on workshops on instruments and simulated systems. The main conference on June 5 provided a platform for users and Beckman Coulter to share and discuss ideas, applications and new developments. more

User Presentations
- Fully Automated Cherry Picking Process by Using Remote Data on a Beckman Biomek® FX platform Noemí Tabanera, Juan B. García, Francisco Asensio, Alicia Robles, Genoveva Mateos, Patricia Prieto, Pilar Ramos, Angeles Redondo, José R. Tormo and Fernando Peláez. more

- Biomek® Enhanced Automated Sample Titration (BEAST), A Fully Automated Method of Compound Titration for Counterscreen Assays Supporting Lead Optimization Projects Juan Bautista García more

- Automating Genetic Diagnostic Services in the English National Health Service Stuart Bayliss. more


Third Party Publications
- Novel Somatic and Germline Mutations in Cancer Candidate Genes in Glioblastoma, Melanoma and Pancreatic Carcinoma A Balakrishnan, et al. Cancer Res 2007;67(8):3545-50. Introduction. ... PCR products were purified using AMPure (Agencourt Bioscience Corp., Beckman Coulter SpA, Milan, Italy)...
View the abstract >>
- High-Throughput Species Identification: From DNA Isolation to Bioinformatics DE Richardsone, et al. Molecular Ecology Notes (2007) ... 3 liquid-handliing robot and used the Genfind kit (Agencourt) ... View the abstract >>
- Casting the Percomorph Net Widely: The Importance of Broad Taxonomic Sampling in the Search for the Placement of Serranid and Percid Fishes WL Smith, et al. DOI: 10.1643/0045-8511(2007)7(35:CTPNWT)2.0.CO;2. ... workstation with minor modifications to the manufacturer's protocol or using AMPure (Agencourt Biosciences) ... View the abstract >>
- Genetic Variants in P-Selectin and C-Reactive Protein Influence Susceptibility to Cognitive Decline After Cardiac Surgery JP Mathew, et al. J Am Coll Cardiol, 2007; 49:1934-1942, doi:10.1016/j.jacc.2007.01.080 (Published online 30 April 2007). MassArray system (Sequenom, San Diego, California) at Agencourt Bioscience Corporation ... View the abstract >>

* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffmann-LaRoche, Ltd.
For Research Use Only; not for use in diagnostic procedures.
For comments or questions about T3 Update, please contact the T3
Editor.
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