Biomek® 3000 and GenPlex™ in Forensic Genetics Tumor Cells

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Stangegaard M, Tomàs C, Frank-Hansen R, Hansen AJ, Børsting C, Morling N
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V’s Vej, DK-2100 Copenhagen, Denmark

SNP genotyping provides a supplement for conventional STR-based kits currently used for human identification. GenPlex (Applied Biosystems (AB) is a SNP genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium [1] plus the amelogenin gender marker [2]. Our objective was to set up, implement and validate a small and affordable, automated liquid handling robot for forensic case work samples (buccal swabs on, FTA® paper and Qiagen purified blood).

The reaction scheme consisted of numerous steps (Figure 1) and was difficult to perform consistently without an automated liquid handler. All steps were performed in 96-well microtitre plates. Automation was accomplished with a Biomek 3000 (Beckman Coulter) automated liquid handler using five in-house developed methods. All methods allowed the user to select the number of subsequent injections on the capillary electrophoresis instrument (AB 3130xl, AB) enabling processing of both partial and full plates.

Figure 1 : The GenPlex Protocol -(click to enlarge)

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[1] Sanchez,J.J. et al. A multiplex assay with 52 single nucleotide polymorphisms for human identification. Electrophoresis 27, 1713-1724 (2006).
[2] Phillips,C. et al. Evaluation of the Genplex SNP typing system and a 49plex forensic marker panel. Forensic Science International: Genetics 1, 180-185 (2007).
[3] Børsting, C. et al. Manuscript in preparation.

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