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Automation of PCR* Purification Using Agencourt® AMPure® Reagents on the Biomek® 3000 Laboratory Automation Workstation from Beckman Coulter

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Li Liu Ph.D., Amy Yoder and Laura Pajak Ph.D., Beckman Coulter Inc.

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Figure 1. The GenomeLab deck configuration is used for an automated method of purification of PCR productsusing the Biomek 3000 workstation. This configuration contains five positions for labware (P1-P5), four manual latch positions for tip boxes (ML1-ML4), a tool rack with gripper (Rack1), two dedicated positions for the Peltier Thermoshaker and Static devices and a disposal position for waste management. A customer provided thermocycler can be placed in position P5.

The reliable and high-quality purification of PCR products is essential for a successful sequencing process, but manual PCR purification is both labor intensive and time consuming. Therefore, we developed an automated method to provide a complete walk-away system for purification of PCR products. The information included in this poster describes the utilization of the Biomek 3000 Laboratory Automation Workstation for the removal of unincorporated dNTPs, primers, salts and other contaminants from PCR products using Agencourt AMPure reagents. The user interface allows the user to customize an individual run by selecting a full or partial 96-well plate and inputting certain reaction parameters.

The information presented here includes the full description of the automated methods and subsequent results. Representative data obtained from the purification of PCR products using this system are shown. Data from capillary sequencing on the CEQ™ 8800 Genetic Analysis System from Beckman Coulter demonstrate the suitability of the purified PCR products for stringent assays such as capillary sequencing.

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Additional Information

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Application System Requirements

Hardware Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	986120	1	Biomek 3000 Laboratory Automation Workstation
Beckman Coulter, Inc.	986146	1	MP200 multi-channel tool
Beckman Coulter, Inc.	609642	1	Gripper tool
Beckman Coulter, Inc.	A26572	1	GeXP Genetic Analysis System
Beckman Coulter, Inc.	987921	1	DTX 880 Multimode Detector

Software Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	719349	1	Biomek Software

Consumables and Reagents

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	609801	1	Plate, Microtiter, Sample, V-bottom thermal, cycler-compatible, polypropylene, 200 ÂµL nomi
Beckman Coulter, Inc.	000130	1	Agencourt AMPure Kit PCR purification system
Beckman Coulter, Inc.	000219	1	Agencourt SPRIPlate® 96R Ring Magnet Plate
Beckman Coulter, Inc.	372790	1	Quarter Modular Reservoir
Beckman Coulter, Inc.	717252	1	AP96 P200 tip box (GLx_AP96_200uL)
Beckman Coulter Inc.	608120	1	GenomeLab DTCS QuickStart Kit

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* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.

§ May use equivalent substitution

For Research Use Only; not for use in diagnostic procedures.

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