Beckman Coulter Application (system and method support)
A Fully Automated High-Throughput Method for the Purification of PCR* Products on the Biomek® FX Utilizing the QIAGEN QIAquick* Robo96 PCR Clean-up Kit
Jason Fawcett, Troy Cook, and Paul W. Diaz, Ph.D.
Beckman Coulter, Inc.
Standard PCR and new PCR techniques have become, and will continue to be, the workhorse of the biomedical industry. Fully automated methods for the massive generation of full length or modified genes, ligationless cloning, and protein expression will depend heavily on reliable high-throughput methods of generating and manipulating PCR products. To provide the foundation for these endeavors we introduce here a fully automated method for PCR product purification on our new liquid handler, the Biomek FX.
Features
- Post PCR cleanup using the QIAGEN QIAquick PCR cleanup kit.
- Automated on Beckman Coulter’s Biomek FX liquid handler.
- Easy and efficient method uses the complete QIAquick kit in a single method (see Method Flowchart on Application Details page).
- Deck setup is under five minutes (see Figure 1 on Application Details page).
- Purify 384 PCR samples in less than 40 minutes.
- Excellent DNA recovery: 1.2mg – 4.5mg using 10µL – 50µl reaction volumes respectively.
- Suitable for downstream processes like restriction digest and DNA sequencing.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
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