Beckman Coulter Application (system and method support)
Automated Methods for PCR* Setup on the Biomek® FX
Lisa Knapp, Jason Fawcett, and Laura Pajak, Ph.D.
Beckman Coulter, Inc.
Since the development of the PCR amplification process, it has been applied to an expanding number of applications in the fields of medicine, forensics, and basic research. Additionally, manual setup of PCR is both tedious and time consuming; each manual transfer step has the potential to introduce another source of contamination. As such, three automated methods for PCR setup, which vary with respect to primer and source DNA location, have been developed on the Biomek FX.
Features
- Three different methods developed to accommodate different user requirements (see Method Flowchart on Application Details page for details).
- Can create up to 11 PCR plates in less than 9 minutes.
- Labware strategically placed on the deck to minimize possible contamination (see Figure 1 on Application Details for deck layout).
- Method is easily modified to suite a variety of needs.
- Uses high density ALPs (Automated Labware Positioners) to maximize throughput.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
©1998 - 2006 Beckman Coulter, Inc.
