Beckman
Coulter Application (system and method support)
A Wizard Approach to Generating High Quality Plasmid DNA on the Biomek® FX Using the WIZARD* SV96 Plasmid DNA Purification System from Beckman Coulter
Monique Mason, John Snider, Graham J. Threadgill, Ph.D., and Laura Pajak,
Ph.D.
Beckman Coulter, Inc.
The demand for increased throughput for molecular techniques, and specifically, DNA purification methods has led to a number of purification protocols on automated platforms. These automated solutions for DNA purification, however, must generate high quality DNA reagents to be suitable for any number of downstream applications including sequencing, PCR* and transfection. To address this need, a Promega Two Plate Plasmid DNA Miniprep Wizard using the Wizard* SV96 reagents was developed for the Biomek FX, a new, automated liquid handler.
Features
- Wizard approach allows complete customization of experimental parameters.
- Processes two bacterial plates simultaneously using vacuum filtration (see Method Flowchart on Application Details page) in 70 minutes.
- Tip wash station to reduce the number to tips needed. See Figure 1 for deck layout on Application Details page.
- An updated manifold collar allows elution into standard 96well plate.
- Method includes a trash ALP for elimination of labware when necessary.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
