The Extract-N-Amp Plant PCR Kits (Product Codes XNAR, XNAPR, and XNAPRG) have been developed for use as a high-throughput system for the rapid extraction and subsequent amplification of genomic DNA from various plant leaves in a 96-well format. The Extract-N-Amp Plant PCR Kits provide a novel extraction system that eliminates the need for long enzymatic digestions and homogenization steps that are not amenable to automation. The XNAR Kit includes a specially formulated Extract-N-Amp PCR ReadyMix* reagent that is a 2x reaction mixture of buffer, salts, dNTPs, and Taq polymerase. The ReadyMix reagent also contains Sigma’s antibody mediated hot start mechanism, JumpStart* Taq polymerase, for highly specific amplification of genomic DNA directly from the extract. The XNAPR Kit includes the REDExtract-N-Amp* PCR ReadyMix reagent containing an inert tracking dye for convenient direct loading of PCR reactions onto agarose gels for analysis. The XNAPRG Kit includes a SYBR* Green Extract-N-Amp PCR ReadyMix reagent for real-time quantitative analysis of the amplified PCR products.

The validated method created for use on the Biomek® FX Liquid Handling Workstation provides a walk-away protocol for all aspects of the Extract-N-Amp Plant PCR Kits.

Extraction and amplification of genomic DNA from plant leaves is accomplished in 4 easy steps:
    1. The Extraction Solution is added to a piece of leaf tissue.
    2. Extracts are incubated for 10 minutes at 85 °C.
    3. The Dilution Solution is added to the extract. Extracts are now stable for at least 6 months         if stored at 2-8 °C.
    4. PCR reactions are set up using 4 µl of the extracts.

In just 20 minutes, the Biomek FX can complete extraction and PCR reaction setup for 96 leaf tissue samples.