Ultracentrifuge
Plasmid Separations in Tabletop Ultracentrifuges Using Fixed-Angle
Rotors
Stephen E. Little
Beckman Coulter, Inc.
The capability of simultaneously processing a number of samples makes the ultracentrifuge the system of choice for isolation of plasmid DNA. With the wide selection of rotors and large range of tube sizes available for tabletop ultracentrifuges, it is possible to isolate plasmid DNA in mini-prep quantities or standard preparative amounts. In addition, tabletop ultracentrifuges can be located conveniently on the laboratory bench where sample preparation occurs.
Features
- Yields of purified plasmid DNA range from 20µg to several mg can be obtained.
- Using the variable speed protocol, visible band separation is achieved in 1.5 hours.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
