eLabNotebook - Promega's MagneSil* Total RNA mini-Isolation System on the Biomek® FX

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Total RNA mini-Isolation Biomek® FX

Chemistry Partner Application

Promega's MagneSil* Total RNA mini-Isolation System on the Biomek® FX

Promega Corporation – www.promega.com

The MagneSil* Total RNA mini-Isolation System^(a) provides a high-throughput 96-well format for fast, simple preparation of intact, purified total RNA from small amounts of cell culture (=1 × 105 tissue culture cells), tissue (=2mg tissue lysate in 100µl), or freshly isolated whole blood (=20µl). The protocol enables manual purification with the use of a multichannel pipettor and plate shaker or highthroughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (=1 × 103 cells) and freshly isolated whole blood (=5µl) may also be performed. Total RNA purification is achieved without the need for vacuum filtration, centrifugation or precipitation. The 96-well total RNA isolation procedure takes about 30 minutes to complete on a liquid-handling workstation.

The MagneSil* Total RNA mini-Isolation System uses the MagneSil* RNA Paramagnetic Particle technology^(b). After sample lysis, nucleic acids are captured by MagneSil* RNA Paramagnetic Particles. The particles are washed and incubated with a DNase Solution to remove contaminating genomic DNA. The DNase is then inactivated, degraded genomic DNA is washed away, and the purified total RNA is eluted in Nuclease-Free Water (Figure 1). Sufficient reagents are supplied to isolate total RNA from four 96-well or 384-well plates. All components are guaranteed free of contaminating ribonucleases when used as directed and are thoroughly tested to ensure optimal performance.

The protocol provided in this technical bulletin provides instructions for automated or manual isolation of total RNA using the MagneSil* Total RNA mini-Isolation System. Specific instructions are provided for the Beckman Coulter Biomek® FX Laboratory Workstation. Information about obtaining automated methods for these workstations is available at: www.promega.com/automethods/

Total RNA purified using the MagneSil* Total RNA mini-Isolation System is suitable for a variety of molecular biology applications including end-point RT-PCR^(c) amplification and real-time RT-PCR.

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.