High-Throughput Genomic DNA Isolation from Cell Cultures and Mouse Tails
on the Biomek® FX Liquid Handling System using Promega's Wizard* SV 96
Genomic DNA Purification System
Hobert Wai, Ph.D.**, Matthew Cu**, Dana P. Campbell**, and Keith Roby**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Terri Grunst and Daniel Kephart
Promega Corporation –
www.promega.com
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717001 | 1 | Biomek FX Hybrid System |
| Beckman Coulter, Inc. | 719368 | 1 | 96 channel head 200µL |
| Beckman Coulter, Inc. | A16170 | 1 | Biomek System Software and Automation Controller |
| Beckman Coulter, Inc. | 719356 | 1 | Biomek Disposable Tip Loader |
| Beckman Coulter, Inc. | 719357 | 3 | 1x1 ALP |
| Beckman Coulter, Inc. | 719948 | 1 | 4x3 ALP |
| Beckman Coulter, Inc. | 719400 | 1 | Biomek FX Filtration System (Single Plate) with Vacuum
Valve Unit System |
| Beckman Coulter, Inc. | 717251 | 3/plate | Biomek AP96 P250 Tips, Nonsterile |
Consumables
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 140504 | 1 | Square Well Deep-Well Plate |
| Promega Corporation | A2370 | 1 | Wizard SV 96 Genomic DNA Purification System, 1x96 |
| Promega Corporation | A2371 | 1 | Wizard SV 96 Genomic DNA Purification System, 4x96 |
| Innovative Microplate | S30014 | 1 | 96-Well Reservoir, Pyramid-Bottom |
Suitable Vacuum Pumps
| Source | Part No. | Quantity | Description |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Nalgene | 2162-0830 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting Closure 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
