Beckman
Coulter Application (system and method support)
High-Throughput Genomic DNA Isolation from Cell Cultures and Mouse Tails on the Biomek® FX Liquid Handling System using Promega's Wizard* SV 96 Genomic DNA Purification System
Hobert Wai, Ph.D.**, Matthew Cu**, Dana P. Campbell**, and Keith Roby**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Terri Grunst and Daniel Kephart
Promega Corporation –
www.promega.com
We have developed an automated method for the isolation of pure genomic DNA from Cell Culture and mouse tails on the Biomek FX to avoid a labor-intensive and time-consuming procedure that has been susceptible to contamination from different sources. Specifically, we have developed multiple automated methods for the isolation of genomic DNA from tissue cell cultures and mouse tail clippings using the Promega Wizard SV 96 Genomic Purification System (Promega, Inc., Madison WI) and Beckman Coulter’s Biomek® FX Liquid Handling System. By using a 96-well vacuum filtration procedure to isolate and purify genomic DNA from tissue cell cultures and mouse tail lysates, the need for centrifugation has been eliminated. Washing the bound genomic DNA requires no disassembly of the vacuum manifold and filtrate waste products are delivered directly to a vacuum trap. Genomic DNA is eluted into a convenient 96-well deep-well plate for convenient downstream sample analysis making the process simple and easy to implement in the genomic research laboratory. The purified genomic DNA has been evaluated for quality, reproducibility, degradation,and cross-contamination using spectrophotometry, agarose gel electrophoresis, and PCR* analysis.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
