High-Throughput Genomic DNA Isolation from Cell Cultures and Mouse Tails
on the Biomek® FX Liquid Handling System using Promega's Wizard* SV 96
Genomic DNA Purification System
Hobert Wai, Ph.D.**, Matthew Cu**, Dana P. Campbell**, and Keith Roby**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Terri Grunst and Daniel Kephart
Promega Corporation –
www.promega.com
Figure 1A. Deck Setup for genomic DNA purification from
one 96-well tissue culture plate.The lids from the tip boxes were used
to hold the SV Lysis Buffer, Wash Solution, and Nuclease-free water for
this method.
Figure 1B. Deck Setup for genomic DNA purification from two 96-well tissue
culture plates. The lysis buffer, wash solution, and nuclease-free water
were stored in 96-well pyramid-bottom reservoirs due to high-volume usage.
The pipette tips for samples 1 and 2 were kept separate to limit cross
contamination.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
