Beckman Coulter Application (system support with verified methods)
Using Beckman Coulter's Biomek® NX Laboratory Automation Workstation for the Purification of High Quality Plasmid DNA
Laura Pajak**, Keith Roby**, Chad Pittman** and Scott Boyer**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
The information included in this poster describes the utilization of
a new automated liquid handler, the Biomek NX Laboratory Automation Workstation,
in the preparation of plasmid DNA using Promega’s Wizard* SV96 Plasmid
DNA Purification System. A single plate of bacterial pellets can be purified
on
the deck of the Biomek NX in approximately 50 minutes. The following system
components, for the purification of plasmids, will be described:
• The hardware requirements for the Biomek NX
• Software and method to drive the automation workstation
• Results when purifying plasmids using Wizard SV96 reagents
Plasmids were analyzed by standard methodologies to assess the quality
and quantity of product. Automated capillary sequencing was performed
on Beckman Coulter’s CEQ? 8000 Genetic Analysis System thereby,
demonstrating the suitability of the purified plasmids for capillary sequencing.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
