Beckman Coulter Application (system support with verified methods)
Plasmid Purification Using Promega's Wizard* SV96 Reagents and Beckman Coulter's Biomek® 3000 Laboratory Automation Workstation
Ruth Zhang**, Laura Pajak**, Chad Pittman** and Scott Boyer**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
The information included in this poster describes the utilization of
a new liquid handler, the Biomek 3000 Laboratory Automation Workstation,
in the preparation of plasmid DNA using Promega’s Wizard SV 96 reagents.
Using this system, plasmids are purified via binding to and eventual elution
from a silica matrix using vacuum filtration. The following system components
for the purification of plasmids will be described:
• The new automated workstation, the Biomek 3000
• The software and method to drive the workstation
• The results when purifying plasmids using Wizard SV 96 reagents
Representative data obtained from the purification of plasmids using this
system will be shown. Data from capillary sequencing on Beckman Coulter’s
CEQTM 8000 Genetic Analysis System will be shown demonstrating the suitability
of the purified plasmids for stringent assays such as capillary sequencing.
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