Highly Precise DNA Sizing on the CEQ™ 2000 Fragment Analysis System
Keith Roby, Mark Dobbs, Doni Clark, Scott Boyer, and Graham Threadgill
Beckman Coulter, Inc.
| Q. | Can I perform DNA sequencing and fragment analysis on the same plate? |
| A. | You may perform both on the same physical
plate, but since two different capillary lengths are required for
the two applications, you must change the capillaries and plenum manually
in between. It is most practical to separate samples for sequencing
into one block of wells and samples for fragment analysis into a second
block of wells. The two blocks of wells can be saved as two non-overlapping
virtual sample plates. Do not type the word “blank” in the blank sample
plate wells – the software will assume that you have a number of samples
called “blank”; just delete all the text and let the sample setup
software do the rest. After the first block is complete, exchange
the capillaries and start the second virtual plate.
NOTE: |
| Q. | Can I switch to another database to inspect old data while new data is being collected? |
| A. | No, you may only retrieve data from the active database, and you may not switch the active database while any of the other CEQ software modules (Run, Sample Setup, or Analysis) is open. However, data from different projects within the same database can be examined freely, even while new data is being collected. |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
