From Tissues to Targets
Introducing a New World of Enhanced Proteomics Applications
The ProteomeLab PA 800 -- and all of our ProteomeLab offerings -- are
designed to help to serve as an extension of your thinking, making it easier for
you to do your best science, your way. That means you get a complete solution
that puts meaningful, actionable information into your hands, quickly and
easily.
By taking full advantage of our expertise in both capillary electrophoresis
and automation technologies, the ProteomeLab PA 800 delivers a comprehensive
in-solution protein characterization (protein electrophoresis) system designed
to optimize proteomics discovery.
The ProteomeLab PA 800 resolves and quantifies proteins by their isoelectric
point and molecular weight, generates high-resolution peptide maps and
carbohydrate profiles and provides front-end separation to mass spectrometry.
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Product Features
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To ensure compatibility with automated
fractionation processes, sampling formats include 96-well plates,
microfuge tubes, PCR* vials and 2 ml vials. |
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From peptides to proteins to
carbohydrates, modular photodiode array and laser-induced fluorescence
detection deliver high-sensitivity, quantitative analysis capabilities. |
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For structural elucidation, the
ProteomeLab PA 800's external detector adapter allows for easy
interfacing of the separation capillary with electrospray mass
spectrometry. |
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Temperature control of the sample
compartment ranges from 5 - 60° C, maintaining protein stability while
enabling temperature impact studies on enzymatic activity and protein
interaction measurements. |
Applications
Protein Sizing by Automated SDS-Gel Electrophoreses
SDS-PAGE is one of the most common methods used to assess the molecular
weight of proteins. The ProteomeLab PA 800 automates the process of molecular
weight-based purity determinations, and with on-line UV detection provides
accurate quantitation without the need for staining. This means that results are
obtained quickly, with no gel preparation required. Samples are treated in the
same manner as SDS-PAGE (heat denaturation in the presence of SDS with optional
disulfide bond reduction). And the separation is by capillary gel
electrophoresis, using a formulation of polymers optimized for the resolution of
proteins with a wide molecular weight range.
Figure A illustrates the analysis of size standards. Figure B highlights the
analysis of a reduced IgG monoclonal antibody (L=light chain, H=heavy chain and
NG=non-glycosylated heavy chain). The inset electropherogram (Figure B)
highlights a non-glycosal heavy chain region.
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To perform both reduced and non-reduced
evaluations of immunoglobulins, the ProteomeLab SDS-MW gel analysis has
been developed into an IgG assay kit. Gel buffer, sample buffer and
control reagents are also available as standalone components for
designing custom analyses of all other proteins. |
Carbohydrate Profiling and Analysis
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The ProteomeLab Carbohydrate Labeling and
Analysis Kit contains the reagents, methods and controls needed for both
oligosaccharide and monosaccharide analysis. |
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The distribution of glycans released from
a glycoprotein yields a fingerprint useful in protein identification.
The ProteomeLab PA 800 provides rapid analysis of released glycans, with
separation times typically less than 15 minutes. Laser-induced
fluorescence provides high sensitivity, enabling you to simply "dilute
and shoot" samples. |
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The analysis of monosaccharide composition
can provide valuable information during the initial characterization of
a glycoprotein. A clear advantage of our ProteomeLab PA 800 approach is
in the specificity of the assay. The reductive amination and open-tube
format allow this analysis to be performed directly from the
glycoprotein hydrolysate--so there's no need for posthydrolysis cleanup.
With LIF and APTS derivization, each sugar yields the same detector
response, such that their relative quantities can be directly compared. |
Resolving Proteins by Charge Characteristics
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By determining a protein's isoelectric
point, identity can be partially established. Heterogeneity can be
assessed with quantitation of the different variants separated. Along
with speed and automation advantages, the ProteomeLab PA 800 automated
capillary isoelectric focusing (CIEF) offers direct quantification with
on-line UV detection. |
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The solution-based approach of the
ProteomeLab PA 800 allows easy creation of an environment to assess
differences in a protein's glycosylation state. By doing this directly
from the protein -- through the addition of salts like borate --
complexes can be formed which amplify the charge state of the
glycovariants. This is the simplest approach to assessing the
heterogeneity of a glycoprotein. |
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The ProteomeLab Methods Development kit
provides chemistry for the analysis of proteins which are highly basic
or acidic. This chemistry allows you to resolve proteins by difference
in mass/charge. The kit includes buffers, controls and a specialized
coated capillary to minimize surface interactions. |
Automated Peptide Mapping
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The ProteomeLab PA 800 is optimized for
high-resolution peptide mapping, utilizing detection either by UV or
through external interface to mass spectrometry (MS). By incorporating
the separation prior to MS, sensitivity is increased. Plus, enhanced
information on the mobility of each peptide is obtained, which can be
useful in elucidating the identity of each species. The two
electropherograms below illustrate the analysis of tryptic digests
Cyctochrome C from two different species. Clear differences can be
identified via the PA 800 and characterized further by MS. |
32 Karat™ Software
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Our powerful and intuitive 32 Karat software delivers
fast, easy automated application setup. |
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Automated fraction collection allows isolation of
protein components at very low levels for activity measurement, or MALDI
plate spotting. |
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32 Karat software contains the technical controls
necessary for compliance with Title 21 CFR Part 11, including system,
method, data and sequence audit trails, permanent read-only method
storage, and user-authentication routines with levels access for
electronic signature signoff. |
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![eLabNotebook[TM]](/products/images/analytical/protein/pf2d_eln.jpg)
