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Convenient Packaging of DNA Synthesis Reagents
The dramatic increase in the demand for synthetic oligonucleotides has driven the design and construction of high-throughput DNA synthesizers into many different configurations. This increased demand for oligonucleotides has also required that DNA synthesizers be fitted with large capacity bottles for the reagents.
Beckman Coulter has responded to this demand by offering DNA synthesis chemistries in bottles of both standard and a variety of bulk formats. Listed in the right-hand column of the table "Liquid DNA Synthesis Reagents in Large Bottle" are examples of our non-standard packaging. For standard format information, contact your local Beckman Coulter representative.
Controlled Pore Glass (CPG) with Attached Nucleotides
- The titer for the attached nucleotide is in the range of 20 - 30 micromoles / milligram of CPG.
- The CPG particle size is 75 mm.
- The CPG is available in 1,000 Angstroms pore size.
| Part Number |
Description |
| 141420 |
dA(Bz) - CPG |
| 338493 |
dC(Ac) - CPG |
| 141421 |
dC(Bz) - CPG |
| 141422 |
dG(iBu) - CPG |
| 141423 |
dT - CPG |
| 608662* |
Universal CPG |
* Specific Cleavage and Deprotection reagent required, P/N 608663.
CPG - nucleotide is ordered in multiple units of 1 gram. The CPG - nucleotide is delivered in user-defined containers, usually 20 mL amber glass bottles or large plastic containers.
Cyanoethyl - Phosphoramidite, Bulk Format:
1 gram units in user-defined quantities and containers
| Part Number |
Description |
Purity by Reverse Phase HPLC |
Coupling Efficiency |
| Ultra Pure |
|
|
|
| 338502 |
dA(Bz) |
>98% |
>98% |
| 338494 |
dC(Ac) |
>98% |
>98% |
| 338503 |
dC(Bz) |
>98% |
>98% |
| 338504 |
dG(iBu) |
>98% |
>98% |
| 338505 |
dT |
>98% |
>98% |
| Pure |
|
|
|
| 608665 |
dA(Bz) |
>96% |
>98% |
| 608667 |
dC(Ac) |
>96% |
>98% |
| 608666 |
dC(Bz) |
>96% |
>98% |
| 608668 |
dG(iBu) |
>96% |
>98% |
| 608669 |
dT |
>96% |
>98% |
Order these CE-phosphoramidites in multiples of 1-gram units. These CE-phosphoramidites are delivered in user defined glass bottles. Quantities of 100 grams and above are delivered in plastic containers.
Liquid DNA Synthesis Reagents in Large Bottle:
| Part Number |
Description |
Quantity |
| 608661 |
Oxidizer (iodine solution in THF) |
450 mls |
| 608657 |
Deblock (trichloracetic acid in dichlormethane) |
450 mls |
| 608659 |
Cap 1 (acetic anhydride in THF/pyridine/water) |
450 mls |
| 608660 |
Cap 2 (N-methyl imidazole) |
450 mls |
| 608658 |
Activator (tetrazole in acetonitrile) |
450 mls |
| 608633 |
Oxidizer (iodine solution in THF) |
950 mls |
| 608629 |
Deblock (trichloracetic acid in dichlormethane) |
950 mls |
| 608631 |
Cap 1 (acetic anhydride in THF/pyridine/water) |
950 mls |
| 608632 |
Cap 2 (N-methyl imidazole) |
950 mls |
| 608630 |
Activator (tetrazole in acetonitrile) |
950 mls |
Pricing:
For all items listed here, please contact your Beckman Coulter representative for pricing.
Certificates of Analysis:
A Certificate of Analysis can be obtained for any of these reagents upon request.
Custom Packaging:
If a bottling requirement is not met by any of the above configurations, please contact your Beckman Coulter representative and inquire about our custom packaging program.
Abbreviations: (Bz) - Benzoyl, (iBu) - isobutryl, (Ac) - acetyl
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